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microarray hybridization chamber  (Camlab Ltd)

 
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    Camlab Ltd microarray hybridization chamber
    Microarray Hybridization Chamber, supplied by Camlab Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+chambers/hybridization+chamber/pm40561749-99-7-10
    Average 90 stars, based on 1 article reviews
    microarray hybridization chamber - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Sterility:

    Article Title: Bradykinin stimulates cell proliferation through an extracellular-regulated kinase 1 and 2-dependent mechanism in breast cancer cells in primary culture.
    Article Snippet: .. The slides were covered with a sterile coverglass and incubated at 42 C for 15–18 h in microarray hybridisation chambers (Camlab, Cambridge, Cambs, UK). .. The slides were then washed in 1 SSC, 0·2% SDS, then in 0·1 SSC and 0·2% SDS and finally in 0·1% SSC and centrifuged in a Falcon tube at 800 g for 5 min to dry.

    Incubation:

    Article Title: Bradykinin stimulates cell proliferation through an extracellular-regulated kinase 1 and 2-dependent mechanism in breast cancer cells in primary culture.
    Article Snippet: .. The slides were covered with a sterile coverglass and incubated at 42 C for 15–18 h in microarray hybridisation chambers (Camlab, Cambridge, Cambs, UK). .. The slides were then washed in 1 SSC, 0·2% SDS, then in 0·1 SSC and 0·2% SDS and finally in 0·1% SSC and centrifuged in a Falcon tube at 800 g for 5 min to dry.

    Microarray:

    Article Title: Bradykinin stimulates cell proliferation through an extracellular-regulated kinase 1 and 2-dependent mechanism in breast cancer cells in primary culture.
    Article Snippet: .. The slides were covered with a sterile coverglass and incubated at 42 C for 15–18 h in microarray hybridisation chambers (Camlab, Cambridge, Cambs, UK). .. The slides were then washed in 1 SSC, 0·2% SDS, then in 0·1 SSC and 0·2% SDS and finally in 0·1% SSC and centrifuged in a Falcon tube at 800 g for 5 min to dry.

    Hybridization:

    Article Title: Bradykinin stimulates cell proliferation through an extracellular-regulated kinase 1 and 2-dependent mechanism in breast cancer cells in primary culture.
    Article Snippet: .. The slides were covered with a sterile coverglass and incubated at 42 C for 15–18 h in microarray hybridisation chambers (Camlab, Cambridge, Cambs, UK). .. The slides were then washed in 1 SSC, 0·2% SDS, then in 0·1 SSC and 0·2% SDS and finally in 0·1% SSC and centrifuged in a Falcon tube at 800 g for 5 min to dry.



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    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the <t>microarray</t> experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).
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    Image Search Results


    Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Journal: BMC Cancer

    Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia

    doi: 10.1186/1471-2407-13-364

    Figure Lengend Snippet: Mature miR- 221 is overexpressed in AML. 22 blast-enriched AML samples and 9 healthy controls (3 CD34+, 3 BM, 3 PB), all of which had also been included in the microarray experiments, were subjected to Taqman qRT-PCR to measure mature miR-221 (A) or miR-222 (B) . Expression values were calculated according to the ΔΔCt method , with RNU6B as internal reference and PB from healthy donor A as calibrator. *, p < 0.05; n.s., not significant (miR-222, p = 0.064).

    Article Snippet: Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA).

    Techniques: Microarray, Quantitative RT-PCR, Expressing